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人结肠腺癌细胞LS 174T
  【编号】:LS 174T

  【名称】:人结肠腺癌细胞LS 174T

  【规格】:株

  【价格】:1000

  LS 174T人结肠腺癌细胞

  描述:LS 174T是LS 180 (ATCC CL 187)结肠腺癌细胞株的胰蛋白酶化变种。 它比亲本更易传代,象LS 180一样生成大量的癌胚抗原(CEA)。 电镜研究表明有丰富的微丝和细胞质粘液素液泡。 直肠抗原3阳性。 p53抗原表达阴性,但mRNA表达阳性。 与ATCC CL-187来源于同一个肿瘤。LS 174T细胞角蛋白染色阳性。 癌基因c-myc, N-myc, H-ras, N-ras, Myb, 和 fos的表达呈阳性。 癌基因k-ras和sis的表达未做检测。
  动物种别:人
  性别:女
  组织来源:直肠;结直肠腺癌
  形态:上皮细胞
  培养基和添加剂:DMEM培养基(GIBCO,货号12800017,添加NaHCO3 1.5g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
  供应限制:A。仅供研究之用。
  REFERENCE:Tom BH , et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041 Jakstys MM , et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980 Chen TR , et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 Gastl GA , et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757 Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 Tsao H , et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 Shibata D , et al. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat. Genet. 6: 273-281, 1994. PubMed: 8012390 Kutchera W , et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996.
  中文名称:人结肠腺癌细胞
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