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核酸酶S1 来源于米曲霉, ≥100,000-500,000 units/ml
  【编号】:128634

  【产品名称】:核酸酶S1 来源于米曲霉, ≥100,000-500,000 units/ml

  【规格】:10KU

  【用途】:

  核酸酶S1 来源于米曲霉, ≥100,000-500,000 units/ml

  Product Name: Nuclease S1 from Aspergillus oryzae
  CAS号:37288-25-8
  介绍: Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.
  用途: Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.
  贮存: 储存温度 -20°C
  生化和生理学机理: Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.
上一篇:神经氨酸酶 来源于产气荚膜梭菌, ≥0.5 units/mg dry weight 下一篇:微球菌核酸酶 来源于金黄色葡萄球菌(菌株ATCC #27735), ≥6,000 units/mg pro



    
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