白介素2可溶性受体(人类,rDNA衍生)
【编号】:NIBSC97/600 【名称】:白介素2可溶性受体(人类,rDNA衍生) 【规格】:个 【价格】:2200元 白介素2可溶性受体(人类,rDNA衍生)
编号:NIBSC97/600
英文:Interleukin-2 Soluble Receptor (human, rDNA derived)
标准类型: 非WHO参考资料
规格:1个
描述:1. INTENDED USE
2. CAUTION
This preparation is not for administration to humans or animals in
the human food chain.
The preparation contains material of human origin, and either the final
product or the source materials, from which it is derived, have been
tested and found negative for HBsAg, anti-HIV and HCV RNA. As with
all materials of biological origin, this preparation should be regarded as
potentially hazardous to health. It should be used and discarded
according to your own laboratory's safety procedures. Such safety
procedures should include the wearing of protective gloves and
avoiding the generation of aerosols. Care should be exercised in
opening ampoules or vials, to avoid cuts.
3. UNITAGE
The assigned potency is 1000 units of immunological activity per
ampoule.
4. CONTENTS
Country of origin of biological material: United Kingdom.
Each ampoule contains a freeze dried residue comprising, under an
atmosphere of nitrogen:
IL-2sr approximately 30 - 100 ng
9.0 mg sodium chloride
1.0 mg trehalose
2.0 mg human serum albumin
The IL-2sr protein was expressed in CHO cells.
NOTE: Due to the variation in estimates provided by contributors to an
international collaborative study of the mass of IL-2sr contained within the
ampoules, a unitage has been applied to the contents and a range
provided for the mass content. It is strongly recommended that all
immunoassays are calibrated in this unitage.
5. STORAGE
For economy of use, it is recommended that the solution be sub divided
into several small aliquots and stored at -40ºC or below. Avoid repeated
thawing/freezing. Unopened ampoules should be stored at -20ºC.
Please note: because of the inherent stability of lyophilized material,
NIBSC may ship these materials at ambient temperature.
6. DIRECTIONS FOR OPENING
DIN ampoules have an 'easy-open' coloured stress point, where the
narrow ampoule stem joins the wider ampoule body. Various types of
ampoule breaker are available commercially. To open the ampoule,
tap the ampoule gently to collect material at the bottom (labelled) end
and follow manufactures instructions provided with the ampoule
breaker.
7. USE OF MATERIAL
No attempt should be made to weigh out any portion of the freeze-dried
material prior to reconstitution
Dissolve the total contents of the ampoule in 0.5ml of sterile distilled
water. Rinse the ampoule with about 0.4ml of sterile distilled water and
make up the total volume to 1.0ml with distilled water. This solution will contain IL-2 sr at a concentration of 1000 units/ml. Use carrier protein where
extensive dilution is required.
8. STABILITY
Reference materials are held at NIBSC within assured, temperaturecontrolled
storage facilities and they should be stored on receipt as
indicated on the label. An arbitrary unitage was assigned to this
preparation at the time of production without confirmation of long term
stability. Evidence from similar materials prepared by an equivalent
process indicates that long term stability is likely to be maintained and the
material is suitably stable for shipment at ambient temperature without
any effect on the assigned values. Once reconstituted, diluted or
aliquoted, users should determine the stability of the material according
to their own method of preparation, storage and use. Users who have
data supporting any deterioration in the characteristics of any reference
preparation are encouraged to contact NIBSC.
9. REFERENCES
This standard was produced under WHO guidelines cited in the WHO
Technical Reports Series, No. 800, 1990, Annex 4.
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