白介素3（人类，rDNA衍生）世卫组织国际标准

  【编号】：NIBSC91/510

  【名称】：白介素3（人类，rDNA衍生）世卫组织国际标准

  【规格】：1个

  【价格】：2600元

　　白介素3（人类，rDNA衍生）世卫组织国际标准

　　编号：NIBSC91/510

　　英文：Interleukin-3 (Human, rDNA derived) WHO International Standard

　　标准类型：国际标准

　　规格：1个

　　描述：1. INTENDED USE

This International Standard is the primary biological standard for IL3.

2. CAUTION

This preparation is not for administration to humans or animals in

the human food chain.

The preparation contains material of human origin, and either the final

product or the source materials, from which it is derived, have been

tested and found negative for HBsAg, anti-HIV and HCV RNA. As with

all materials of biological origin, this preparation should be regarded as

potentially hazardous to health. It should be used and discarded

according to your own laboratory's safety procedures. Such safety

procedures should include the wearing of protective gloves and

avoiding the generation of aerosols. Care should be exercised in

opening ampoules or vials, to avoid cuts.

3. UNITAGE

The assigned potency agreed on the basis of WHO International

Collaborative Study is 1700 International Units of biological activity per

ampoule.

4. CONTENTS

Country of origin of biological material: United Kingdom.

Each ampoule contains a freeze dried residue comprising, under an

atmosphere of nitrogen:

IL-3 approximately 1.0 microgram

4.5 mg sodium chloride

0.5 mg trehalose

1.0 mg human serum albumin

The IL-3 protein was expressed in E. coli.

5. STORAGE

For economy of use, it is recommended that the solution be sub divided

into several small aliquots and stored at -40ºC or below. Avoid repeated

thawing/freezing. Unopened ampoules should be stored at -20ºC.

Please note: because of the inherent stability of lyophilized

material, NIBSC may ship these materials at ambient temperature.

6. DIRECTIONS FOR OPENING

Tap the ampoule gently to collect the material at the bottom (labelled)

end. Ensure ampoule is scored all round at the narrow part of the

neck, with a diamond or tungsten carbide tipped glass knife file or

other suitable implement before attempting to open. Place the

ampoule in the ampoule opener, positioning the score at position 'A';

shown in the diagram below. Surround the ampoule with cloth or

layers of tissue paper. Grip the ampoule and holder in the hand and

squeeze at point 'B'. The ampoule will snap open. Take care to avoid

cuts and projectile glass fragments that enter eyes. Take care that no

material is lost from the ampoule and that no glass falls into the

ampoule.Side view of ampoule opening device containing an ampoule positioned

ready to open. 'A' is the score mark and 'B' the point of applied pressure.

7. USE OF MATERIAL

No attempt should be made to weigh out any portion of the freeze-dried

material prior to reconstitution

Dissolve the total contents of the ampoule in 0.5ml of sterile distilled water.

Rinse the ampoule with about 0.4ml of sterile phosphate buffered saline

(PBS) and make up the total volume to 1.0ml with PBS. This solution will

contain IL-3 at a concentration of 1700 Units/ml. Use carrier protein where

extensive dilution is required.

8. STABILITY

Reference materials are held at NIBSC within assured, temperaturecontrolled

storage facilities and they should be stored on receipt as

indicated on the label. It is the policy of WHO not to assign an expiry date

to their international reference materials. Accelerated degradation studies

have indicated that this material is suitably stable, when stored at -20ºC

or below, for the assigned values to remain valid until the material is

withdrawn or replaced. These studies have also shown that the material

is suitably stable for shipment at ambient temperature without any effect

on the assigned values. Once reconstituted, diluted or aliquoted, users

should determine the stability of the material according to their own

method of preparation, storage and use. Users who have data

supporting any deterioration in the characteristics of any reference

preparation are encouraged to contact NIBSC.

9. REFERENCES

Mire-Sluis, A. R, Gaines Das, R., Thorpe, R. et al (1996) Implications for the

assay and biological properties of interleukin-3. Results of a WHO

international collaborative study. Journal of Immunological Methods 194, 1-

12.

This standard was produced under WHO guidelines cited in the WHO

Technical Reports Series, No. 800, 1990, Annex 4.

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